FIGURE

Fig. 13

ID

ZDB-FIG-100616-104

Publication

Insinna et al., 2010 - Analysis of a zebrafish dync1h1 mutant reveals multiple functions for cytoplasmic dynein 1 during retinal photoreceptor development

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Fig. 13

Efficient transport of Rhodopsin(CT44)-GFP to the outer segment is disrupted with loss of Dync1h1 or Dctn1. Cryosections of 3.5-dpf retina from transgenic Tg(1.3xops:xRhoCT44-GFP) embryos in which the carboxy-terminal 44 amino acids of Rhodopsin are fused with GFP. Localization of GFP was evaluated in photoreceptors from (A) control morphants, (B) cnb mutants, (C) dync1h1 morphants or (D) dctn1a/b morphants. Upper row shows propidium iodide staining to highlight nuclei. Middle row shows RhoCT44-GFP. Bottom row shows color combined images. Arrowheads indicate cells with mislocalized GFP to the cell body region.

Expression Data

Gene:	GFP
Fish:	a125Tg a125Tg + MO1-dync1h1 dync1h1^mw20/mw20; a125Tg a125Tg + MO2-dctn1a + MO2-dctn1b
Knockdown Reagents:	MO1-dync1h1 MO2-dctn1a MO2-dctn1b
Anatomical Terms:	retinal rod cell retinal rod cell photoreceptor outer segment
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	a125Tg + MO1-dync1h1 dync1h1^mw20/mw20; a125Tg a125Tg + MO2-dctn1a + MO2-dctn1b
Knockdown Reagents:	MO1-dync1h1 MO2-dctn1a MO2-dctn1b
Observed In:	photoreceptor cell morphogenesis retinal rod cell photoreceptor outer segment
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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