ZFIN Image: Insinna et al., 2010, Fig. 13

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Figure Caption

Fig. 13 Efficient transport of Rhodopsin(CT44)-GFP to the outer segment is disrupted with loss of Dync1h1 or Dctn1. Cryosections of 3.5-dpf retina from transgenic Tg(1.3xops:xRhoCT44-GFP) embryos in which the carboxy-terminal 44 amino acids of Rhodopsin are fused with GFP. Localization of GFP was evaluated in photoreceptors from (A) control morphants, (B) cnb mutants, (C) dync1h1 morphants or (D) dctn1a/b morphants. Upper row shows propidium iodide staining to highlight nuclei. Middle row shows RhoCT44-GFP. Bottom row shows color combined images. Arrowheads indicate cells with mislocalized GFP to the cell body region.

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Acknowledgments

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