Fig. S1

Müller glia and photoreceptor phenotypes in ako and mok mutants. (A-D′) Confocal images of transverse cryosections through the retinae of mok (B,B′) and ako (D,D′) mutants at 4 dpf. Wild-type siblings of mutant animals are shown to the left (A,A′,C,C′). In all panels, Müller glia are labeled via the expression of the Tg(gfap:GFP) transgene (green). In addition, sections are stained with the Zpr-1 antibody to visualize double cones (red). Photoreceptor loss in ako^jj50 mutant retinae is more severe than in mok^m632. At 4 dpf, the numbers of ako^jj50 and mok^m632 mutant photoreceptors are reduced to 12% (n=46) and 26% (n=99) of the wild-type value (n=379), respectively. Wild-type Müller glia feature perikarya that localize to the vitreal half of the inner nuclear layer, and processes that span the entire thickness of the retina (A,A′,C,C′). In mok^m632 mutants, many Müller glia maintain perikarya in the inner nuclear layer, and feature apical process at 4 dpf (B,B′). By contrast, in the ako^jj50 mutant, almost all glial perikarya are misplaced basally and the apical processes are largely absent (D,D′). A′,B′,C′,D′ present enlargements of the images shown to their left. In all panels, dorsal is up. L, lens. Scale bars: 40 μm in A-D; 8 μm in A′-D′).