FIGURE

Fig. 11

ID

ZDB-FIG-090817-19

Publication

McMahon et al., 2009 - Lmx1b is essential for survival of periocular mesenchymal cells and influences Fgf-mediated retinal patterning in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 11

Lmx1b is required for patterning the retina in a manner partially independent from Fgf signaling. Expression of nasal, temporal, and dorsal ocular markers with reduced Fgf signaling and Lmx1b function. Lateral view of 24 hpf eyes from whole mount in situ hybridization of (panel A) efna5a (nasal), (panel B) eph4b (temporal), and (panel C) tbx5 (dorsal). Note the reduction of efna5a and tbx5 markers in fgf8a/ace mutants or SU5402-treated embryos and expansion of these markers in lmx1b morphants. Partially rescued and intermediate phenotypes were observed when Fgf signaling was reduced in lmx1b morphants. With eph4b, expansion was noted in fgf8a/ace mutants or SU5402-treated embryos, while the opposite was found in lmx1b morphants. Again, intermediate phenotypes were observed when Fgf signaling was reduced in lmx1b morphants.

Expression Data

Genes:	efna5a epha4b tbx5a
Fish:	WT fgf8a^{ti282a/ti282a} WT + MO2-lmx1bb + MO3-lmx1ba fgf8a^{ti282a/ti282a} + MO2-lmx1bb + MO3-lmx1ba
Condition:	chemical treatment: SU5402
Knockdown Reagents:	MO2-lmx1bb MO3-lmx1ba
Anatomical Terms:	eye eye dorsal region
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fgf8a^{ti282a/ti282a} WT + MO2-lmx1bb + MO3-lmx1ba fgf8a^{ti282a/ti282a} + MO2-lmx1bb + MO3-lmx1ba
Condition:	chemical treatment: SU5402
Knockdown Reagents:	MO2-lmx1bb MO3-lmx1ba
Observed In:	retina development in camera-type eye
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 332(2), McMahon, C., Gestri, G., Wilson, S.W., and Link, B.A., Lmx1b is essential for survival of periocular mesenchymal cells and influences Fgf-mediated retinal patterning in zebrafish, 287-298, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.