FIGURE

Fig. 3

ID

ZDB-FIG-090817-11

Publication

McMahon et al., 2009 - Lmx1b is essential for survival of periocular mesenchymal cells and influences Fgf-mediated retinal patterning in zebrafish

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Fig. 3

Lmx1b loss of function induces apoptosis of Lmx1b expressing cells. (A) TUNEL staining of apoptotic cells (dark blue, top row) in lmx1bdMO morphants and lmx1bdMO morphants co-injected with p53MO and analyzed at 36 hpf. Red arrow indicates increased apoptosis in the midbrain–hindbrain boundary. Red arrowheads show increased periocular cell death in lmx1bdMO eyes. (B) Eye morphology in embryos corresponding to the above panel as visualized in the betaactinGFP transgenic line. (C) TUNEL staining (red) in sox10:GFP-positive neural crest cells (green) in wild-type and lmx1bdMO morphant eyes. White dots in B outline the lens.

Expression Data

Gene:	EGFP
Fish:	ir937Tg vu119Tg ir937Tg + MO2-lmx1bb + MO3-lmx1ba vu119Tg + MO2-lmx1bb + MO3-lmx1ba vu119Tg + MO2-lmx1bb + MO3-lmx1ba + MO4-tp53
Knockdown Reagents:	MO2-lmx1bb MO3-lmx1ba MO4-tp53
Anatomical Terms:	eye eye plasma membrane periocular mesenchyme
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-lmx1bb + MO3-lmx1ba WT + MO2-lmx1bb + MO3-lmx1ba + MO4-tp53 ir937Tg + MO2-lmx1bb + MO3-lmx1ba vu119Tg + MO2-lmx1bb + MO3-lmx1ba vu119Tg + MO2-lmx1bb + MO3-lmx1ba + MO4-tp53
Knockdown Reagents:	MO2-lmx1bb MO3-lmx1ba MO4-tp53
Observed In:	eye eye ventral region eye morphogenesis midbrain hindbrain boundary periocular mesenchyme
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 332(2), McMahon, C., Gestri, G., Wilson, S.W., and Link, B.A., Lmx1b is essential for survival of periocular mesenchymal cells and influences Fgf-mediated retinal patterning in zebrafish, 287-298, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.