Fig. S9

Convergent-extension defects in ints5 morphants are rescued by smad1 and smad5 RNA. (A-L) Whole-mount in situ hybridization to detect ntl, dlx3 and hgg1 (red) expression in tailbud (TB, 10 hpf) embryos injected with control morpholinos (A,B), ints5 morpholinos (C,D), smad1 RNA (E,F), smad5 RNA (G,H), ints5 morpholinos with smad1 RNA (I,J), or ints5 morpholinos with smad5 RNA (K,L). Black arrowheads indicate the anterior limit of ntl expression in the midline, and red arrows indicate hgg1 staining in the anterior prechordal plate mesendoderm cells. White arrowheads and dashed lines indicate the distance between the lateral limits of dlx3 expression in the neuroectoderm. A,C,E,G,I,K show lateral views of embryos with dorsal to the right; B,D,F,H,J,L show animal pole views of embryos. (M) Histogram showing percentage of injected embryos with wild-type (WT, blue) or impaired (magenta) cell movement marked by ntl/dlx3/hgg1 expression. n>50 embryos, from three independent injection experiments. Scale bars: 50 μm.