Fig. 11

Cerebellum-like structures. Co-staining of anti-Pvalb7 (green) and anti-Vglut1 (magenta) antibodies in the cerebellum-like structures in the optic tectum and antero-dorsal hindbrain: the TL-SM-type I neuron (A–C) and EG-CC-MON circuitries (D–F). Sagittal sections with dorsal to the top. Pvalb7 (A, D) and Vglut1 (B, E) signals and merged images (C, F) are shown. In the TL-SM-type I neuron circuitry, Pvalb7 is expressed in type I neurons in the stratum fibrosum et griseum superficiale (SFGS) of the torus longitudinalis (TL) and their dendrites in the stratum marginale (SM, J), and Vglut1 was detected in the SM. Note that morphology of type I neurons is different from Purkinje cells in the cerebellum: type I neurons have one long primary dendrite that branches far from the soma. A primary dendrite of some type I neurons is not observed because of the plane and width of the section (A). In the EG-CC-MON circuitry, Pvalb7 was detected in the crest cells in the MON and their dendrites in the CC, and Vglut1 was detected in the CC. Other abbreviations are described in Fig. 1. Scale bars: 20 μm.