FIGURE

Fig. 8

ID

ZDB-FIG-090617-37

Publication

Bae et al., 2009 - Anatomy of zebrafish cerebellum and screen for mutations affecting its development

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Fig. 8

Cerebellovestibular tracts. (A, B) Immunostaining of 7 dpf larval brains with zebrin II. Lateral (A) and ventral (B) views with anterior to the left. (C) Immunostaining of a 30 dpf juvenile fish brain with anti-Pvalb7 antibody. Lateral view with anterior to the left. Cerebellovestibular tracts are indicated by arrowhead. (D, E) Retrograde labeling of cerebellovestibular tracts. A biotin-conjugated neural tracer was injected to the vestibular region (asterisk, D), which includes the descending octaval nucleus, of adult fish. A sagittal section was stained with zebrin II (green) and fluorescently labeled avidin (magenta). (E) High-magnification view of the box in (D). Note that a retrogradely labeled cell is a zebrin II⁺ Purkinje cell. The abbreviations are described in Fig. 1. Scale bars: 500 μm (D), 100 μm (A–C) and 50 μm (E).

Expression Data

Genes:	aldoca pvalb7
Antibody:	Ab1-aldoc
Fish:	WT
Anatomical Terms:	corpus cerebelli brainstem and spinal white matter corpus cerebelli Purkinje cell molecular layer corpus cerebelli molecular layer valvula cerebelli
Stage Range:	Days 7-13 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 330(2), Bae, Y.K., Kani, S., Shimizu, T., Tanabe, K., Nojima, H., Kimura, Y., Higashijima, S.I., and Hibi, M., Anatomy of zebrafish cerebellum and screen for mutations affecting its development, 406-426, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.