Fig. 7

Mossy fibers and efferent fibers from eurydendroid cells. (A) Schematic presentation of mossy fibers and efferent fibers. (B, C) Co-staining of a Tg(vglut2a:EGFP) brain with zn5 (magenta) and anti-EGFP (green) antibodies. (C) High-magnification view of the box in B. The zn5⁺ vglut2a:EGFP⁺ mossy fibers are indicated by an arrow. (D, E) Expression of alcam (zn5). (E) High-magnification view of the box in D. The alcam-expressing cells (marked by an arrow) are located in the vicinity of the trochlear nerve (IV, marked by an arrowhead). (F, G) Co-staining of the Tg(vglut2a:EGFP) brain with anti-calretinin (magenta) and anti-EGFP (green) antibodies. (G) High-magnification view of the box in F. The anti-calretinin antibody stained the soma (arrowheads) and efferent axons (arrow) of eurydendroid cells (vglut2a:EGFP⁺). (H, I) Co-staining of a Tg(pou4f1-hsp70l:EGFP) brain with anti-calretinin (magenta) and anti-EGFP (green) antibodies. All of the pou4f1-hsp70l:EGFP⁺ axons were stained with the anti-calretinin antibody (arrowheads, climbing fibers). Calretinin-immunoreactive pou4f1-hsp70l:EGFP^- tracts (arrows) are the axons of eurydendroid cells. F/G and H/I show mediolateral and lateral sections, respectively. The abbreviations are described in Fig. 1. Scale bars: 200 μm (B, D, E, F, H, J), 50 μm (G), and 20 μm (C, I).