FIGURE

Fig. 2

ID

ZDB-FIG-090519-22

Publication

Petko et al., 2009 - Proteomic and functional analysis of NCS-1 binding proteins reveals novel signaling pathways required for inner ear development in zebrafish

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Fig. 2

GST-pulldowns. GST pulldowns were performed to validate NCS-1/NBP interactions. A human NCS-1-GST fusion protein was used to pull down S-tagged zebrafish NBPs. In each panel, the left (+) lane contains a bacterial lysate expressing the designated S-tagged NBP, the middle lane (PD), contains NBPs pulled down from bacterial lysates after incubation with the NCS-1-GST fusion protein, and the right lane (-) shows proteins pulled down from lysates after incubation with GST alone. Blots were probed with an HRP-conjugated S-tag antibody to detect each S-tagged NBP. Molecular weights (kDa) of S-tagged NPB fragments are indicated to the left of each panel.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Neurosci.