FIGURE

Fig. 3

ID

ZDB-FIG-090519-23

Publication

Petko et al., 2009 - Proteomic and functional analysis of NCS-1 binding proteins reveals novel signaling pathways required for inner ear development in zebrafish

Other Figures

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Fig. 3

Knockdown of arf-1, pi4kβ, and dan mRNA expression. Antisense MOs targeted against arf-1, pi4kβ, or dan mRNA were injected into single cell embryos, and morphants analyzed at 48 (C-H) and 72 hpf (K-P). For comparison, control (buffer injected) embryos are shown at 48 (A-B) and 72 (I-J) hpf. Embryos were injected with either 4 ng pi4kβ-ATG MO (C-D, K-L), 2 ng arf1-UTR MO (E-F, M-N), or 1ng of dan-ATG MO (G-H, O-P). Images of otic vesicle (B, D, F, H, J, L, N, P) are all lateral views with anterior to the left. Lateral views of wild-type embryos are shown at 48 (A) and 72 (I) hpf. Lateral views of morphant embryos are shown at 48 (C, E, G) and 72 (K, M, O) hpf.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-pi4kb WT + MO2-arf1 WT + MO2-nbl1
Knockdown Reagents:	MO1-pi4kb MO2-arf1 MO2-nbl1
Observed In:	eye post-vent region semicircular canal semicircular canal development whole organism
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Neurosci.