ZFIN Image: Petko et al., 2009, Fig. 2

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Figure Caption

Fig. 2 GST-pulldowns. GST pulldowns were performed to validate NCS-1/NBP interactions. A human NCS-1-GST fusion protein was used to pull down S-tagged zebrafish NBPs. In each panel, the left (+) lane contains a bacterial lysate expressing the designated S-tagged NBP, the middle lane (PD), contains NBPs pulled down from bacterial lysates after incubation with the NCS-1-GST fusion protein, and the right lane (-) shows proteins pulled down from lysates after incubation with GST alone. Blots were probed with an HRP-conjugated S-tag antibody to detect each S-tagged NBP. Molecular weights (kDa) of S-tagged NPB fragments are indicated to the left of each panel.

Acknowledgments

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