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Initiation of gata4, gata5 and gata6 expression is independent of scl and cloche. (A) Expression of the GATA factors was analysed in scl morphants. gata4, gata5 and gata6 were expressed as in the wild-type embryos in the ALM (red arrowheads), placing them upstream of scl. Loss of pu1 was used as a control for scl morpholino efficacy. (B) The expression of gata4, gata5 and gata6 in the ALM was unchanged (red arrowheads) in cloche embryos placing the GATA factors parallel to or upstream of cloche. (C) cloche embryos were identified by the absence of gata1 expression (red arrowheads) in the PLM. Pu1 downregulation was an additional control (B). The expression of uncx4.1 was used as a somitic counter to ensure correct staging of the embryos (white brackets). Views are anterior-dorsal. For each gene analysed, n=28-42, and the images shown depict the findings for >95% of the embryos identified as cloche and scl morphant embryos.
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