FIGURE

Fig. S4

ID

ZDB-FIG-081209-11

Publication

Schenck et al., 2008 - The endosomal protein Appl1 mediates Akt substrate specificity and cell survival in vertebrate development

Other Figures

All Figure Page

Back to All Figure Page

Fig. S4

Concurrent downregulation of p53 does not affect APPL1 knock-down induced apoptosis.
Forebrain apoptosis assay: Quantitative evaluation of apoptosis in appl1 morphants (8 ng MO1A) in which unspecific cell death is blocked by concurrent knock-down of p53 (MO1A + MOp53: 8 ng MO1A + 4 ng MOp53). Embryos were assigned to one of four cell death categories: wt, moderate, very strong forebrain and systemic apoptosis. 5 independent experiments were carried out. Evaluation of Appl1 knockdown (MO1A: 8 ng MO1A) embryos (presented in Figure 4G) is shown again for comparison. Total amount of embryos scored: n^MO1A= 532, n ^MO1A+Mop53= 486. Error bars indicate standard deviation between experiments. All experiments have been carried out on 54 h zebrafish embryos. Efficient knock-down of p53 by 2 ng of the utilized MOp53 has been previously demonstrated (Nowak et al., 2005).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-appl1 WT + MO1-appl1 + MO1-tp53
Knockdown Reagents:	MO1-appl1 MO1-tp53
Observed In:	forebrain
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Cell, 133(3), Schenck, A., Goto-Silva, L., Collinet, C., Rhinn, M., Giner, A., Habermann, B., Brand, M., and Zerial, M., The endosomal protein Appl1 mediates Akt substrate specificity and cell survival in vertebrate development, 486-497, Copyright (2008) with permission from Elsevier. Full text @ Cell