FIGURE

Fig. S3

ID

ZDB-FIG-080508-12

Publication

Schenck et al., 2008 - The endosomal protein Appl1 mediates Akt substrate specificity and cell survival in vertebrate development

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Fig. S3

appl1 morphants show no significant differences in cell proliferation.
(A) Zebrafish embryos injected with injection buffer only (MOCK), 8 ng („low dose“) or 12 ng („high dose“) MO1A, immunostained with anti-P-Histone H3 antibodies (in purple) to highlight mitotic cells. Top and side views of heads and side views of tails are shown (from the left to the right). Scale bar: 250 μm.
(B) Quantification of anti-P-Histone H3 labeled cell in zebrafish heads (including midbrain hindbrain boundary) as shown in (A), left panels. 5 heads per genotype have been evaluated. Error bars indicate standard deviation. Despite the occurring apoptosis (see Figure 4), no significant changes in the number of mitotic cells were found (P values > 0,15).

Expression Data

Antibody:	Ab4-h3
Fish:	WT WT + MO1-appl1
Knockdown Reagent:	MO1-appl1
Anatomical Term:	whole organism
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-appl1
Knockdown Reagent:	MO1-appl1
Observed In:	cell population proliferation
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 133(3), Schenck, A., Goto-Silva, L., Collinet, C., Rhinn, M., Giner, A., Habermann, B., Brand, M., and Zerial, M., The endosomal protein Appl1 mediates Akt substrate specificity and cell survival in vertebrate development, 486-497, Copyright (2008) with permission from Elsevier. Full text @ Cell