ZFIN Image: Schenck et al., 2008, Fig. S4

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Figure Caption

Fig. S4 Concurrent downregulation of p53 does not affect APPL1 knock-down induced apoptosis.
Forebrain apoptosis assay: Quantitative evaluation of apoptosis in appl1 morphants (8 ng MO1A) in which unspecific cell death is blocked by concurrent knock-down of p53 (MO1A + MOp53: 8 ng MO1A + 4 ng MOp53). Embryos were assigned to one of four cell death categories: wt, moderate, very strong forebrain and systemic apoptosis. 5 independent experiments were carried out. Evaluation of Appl1 knockdown (MO1A: 8 ng MO1A) embryos (presented in Figure 4G) is shown again for comparison. Total amount of embryos scored: n^MO1A= 532, n ^MO1A+Mop53= 486. Error bars indicate standard deviation between experiments. All experiments have been carried out on 54 h zebrafish embryos. Efficient knock-down of p53 by 2 ng of the utilized MOp53 has been previously demonstrated (Nowak et al., 2005).

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Acknowledgments

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Reprinted from Cell, 133(3), Schenck, A., Goto-Silva, L., Collinet, C., Rhinn, M., Giner, A., Habermann, B., Brand, M., and Zerial, M., The endosomal protein Appl1 mediates Akt substrate specificity and cell survival in vertebrate development, 486-497, Copyright (2008) with permission from Elsevier. Full text @ Cell