FIGURE

Fig. 5

ID

ZDB-FIG-080919-9

Publication

Cretekos et al., 1999 - alyron, an insertional mutation affecting early neural crest development in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Analysis of rostrocaudal (A–D) and dorsoventral (E–J) patterning in the neuraxis. Prior to 24 h, aln mutant embryos were identified by simultaneous staining for trp-2 expression, which is greatly reduced in the mutant retina (C, D, G, H). Expression of zash-1A in WT sibling (A) and aln mutant (B) 24-h embryos. The major compartments of the brain are indicated by brackets: telencephalon (t), diencephalon (d), and mesencephalon (m). The hypothalamus (∧), epiphysis (*), and tegmentum (♦) are also indicated. (C, D) Expression of krox-20 in rhombomeres 3 and 5 of the hindbrain in WT sibling (C) and aln mutant (D) 18.5-h embryos, double stained with trp-2 (indicated in C). (E, F) Expression of sonic hedgehog in the ventral brain and floor plate of WT sibling (E) and aln mutant (F) 24-h embryos. (G, H) Expression of c-ret in primary motoneurons in the ventral spinal cord of WT sibling (G) and aln mutant (H) 18-h embryos, double stained with trp-2. (I, J) Transverse sections through the midtrunk of WT sibling (I) and aln mutant (J) embryos stained for isl-2 expression at 24 h. Rohon-Beard primary sensory neurons (rb) and primary motoneurons (pmn) appear normal with respect to location within the neural keel, cell body number, and expression level of isl-2.

Expression Data

Genes:	ascl1a dct egr2b isl2a ret shha
Fish:	EKW paf1^z12Tg/z12Tg (EKW)
Anatomical Terms:	brain ventral region diencephalon epiphysis floor plate hypothalamus midbrain optic vesicle primary motor neuron rhombomere 3 rhombomere 5 Rohon-Beard neuron tegmentum telencephalon
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	paf1^z12Tg/z12Tg (EKW)
Observed In:	neural tube
Stage Range:	14-19 somites to Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 210(2), Cretekos, C.J. and Grunwald, D.J., alyron, an insertional mutation affecting early neural crest development in zebrafish, 322-338, Copyright (1999) with permission from Elsevier. Full text @ Dev. Biol.