FIGURE

Fig. 1

ID

ZDB-FIG-080919-4

Publication

Cretekos et al., 1999 - alyron, an insertional mutation affecting early neural crest development in zebrafish

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Fig. 1

Overview of aln mutant phenotype. (A, B) WT sibling (A) and aln mutant (B) 24-h embryos. aln mutants display delayed pigmentation in the eye, a smaller than normal otic vesicle, and an enlarged pericardial cavity. In addition, mutant embryos are slightly delayed developmentally, having on average two less somites than their WT siblings at 24 h (not shown). (C, D) WT sibling (C) and aln mutant (D) 48-h embryos. aln mutants lack body pigmentation, do not circulate blood, and usually have reduced eye pigmentation. Differentiated blood cells accumulate above the yolk cell in mutants (arrowhead in D), whereas they are visible flowing into the inflow tract of the heart in WT embryos (arrowhead in C). (E, F) Expression of trp-2 in WT sibling (E) and aln mutant (F) 26-h embryos detected by RNA in situ hybridization. At 26 h, trp-2 expression is detectable in the retina but not elsewhere in aln mutants. (G, H) Xanthophores, identified by their bright blue autofluorescence under DAPI epifluorescence, are seen on the dorsal surface of the head of WT 48-h embryos (G), but are not detected in aln mutants (H).

Expression Data

Gene:	dct
Fish:	EKW paf1^z12Tg/z12Tg (EKW)
Anatomical Term:	presumptive retinal pigmented epithelium
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	paf1^z12Tg/z12Tg (EKW)
Observed In:	blood circulation embryonic organ development eye pigment cell eye pigmentation melanocyte otic vesicle pericardial cavity somite trunk xanthophore
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 210(2), Cretekos, C.J. and Grunwald, D.J., alyron, an insertional mutation affecting early neural crest development in zebrafish, 322-338, Copyright (1999) with permission from Elsevier. Full text @ Dev. Biol.