FIGURE

Fig. 7

ID

ZDB-FIG-070421-12

Publication

Pauls et al., 2007 - Function and regulation of zebrafish nkx2.2a during development of pancreatic islet and ducts

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Fig. 7

The development of the pancreatic ducts in nkx2.2a- and ptf1a-morphants. (A, B, D) Lateral views, anterior to the right. (C, E, F) Ventral views, anterior to the left. (A, E) Control embryos. (B, C) nkx2.2a-morphant larvae and (D, F) ptf1a-morphants. (A–D) GFP fluorescence in larvae of the nkx2.2(3.5):GFP line at 4 dpf. (A–D) The position of the intrapancreatic duct, which is lacking in panels B–D is indicated by an arrowhead. (C) Confocal image of a nkx2.2a-morphant larva where the extrapancreatic duct is marked by an arrow. A rudimentary duct is pointing in an anterior direction. (E, F) 48 hpf, putative precursors of the extrapancreatic duct express nkx2.2a (arrow) whereas there is a reduced number of nkx2.2a-expressing cells in the medial portion of the nkx2.2a expression domain (this area is marked by a dashed circle).

Expression Data

Gene:	nkx2.2a
Fish:	WT WT + MO2-ptf1a + MO3-ptf1a
Knockdown Reagents:	MO2-ptf1a MO3-ptf1a
Anatomical Term:	pancreas
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-nkx2.2a WT + MO2-ptf1a + MO3-ptf1a WT + MO3-nkx2.2a
Knockdown Reagents:	MO2-nkx2.2a MO2-ptf1a MO3-nkx2.2a MO3-ptf1a
Observed In:	pancreatic duct
Stage:	Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 304(2), Pauls, S., Zecchin, E., Tiso, N., Bortolussi, M., and Argenton, F., Function and regulation of zebrafish nkx2.2a during development of pancreatic islet and ducts, 875-890, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.