- Title
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Specialized neurons in the right habenula mediate response to aversive olfactory cues
- Authors
- Choi, J.H., Duboue, E.R., Macurak, M., Chanchu, J.M., Halpern, M.E.
- Source
- Full text @ Elife
Iratd2a-expressing neurons in the right dHb connect asymmetric pathway from the olfactory bulb to ventral IPN. |
Targeted genomic integration does not disrupt endogenous gene expression. |
Asymmetry of lratd2a-expressing dHb neurons.(A) Dorsal views of the habenular region of three Tg(lratd2a:QF2); Tg(QUAS:GFP) larvae at 7 dpf. Each confocal image is a single focal plane (1 μm). Brackets indicate regions of lratd2a-expressing neurons in the dHb and arrowheads point to the bilateral vHb. Scale bar, 25 μm. (B) Quantification of lratd2a-expressing dHb neurons. Each line connecting the circles corresponds to a single larva. Mean is 13.1 ± 1.4 cells in the left dHb and 33.5 ± 2.3 cells in the right, n = 15 larvae [p < 0.0001, paired t-test]. |
lhx2a-expressing olfactory cells innervate lratd2a dHb neurons. |
Increased activity of lratd2a-expressing dHb neurons upon exposure to aversive olfactory cues. |
Synaptic inhibition of lratd2a right dHb neurons attenuates response to cadaverine. |
Preferred tank location prior to and after cadaverine addition within each group and between groups. |
Validation of intersectional strategy to inhibit cholinergic neurons using botulinum neurotoxin. |
Variability in BoTxBLC-GFP labeling of dHb neurons. |
Aversive response to alarm substance is intact in BoTxBLC-GFP juvenile fish. |
Attenuated response to aversive odorants by left-isomerized dHb mutants. |
Preferred tank location prior to and after cadaverine addition within each group and between groups. |
Enhanced reactivity to alarm substance in mutants with right-isomerized dHb. |
Preferred tank location prior to and after cadaverine addition within each group and between groups. |