Figure 3—figure supplement 1

(A) Preferred tank location prior to and after cadaverine addition of adults genotyped for absence (Cre-, blue) or presence (Cre+, red) of Tg(slc5a7a:Cre). Preference index for all adults tested 5 min prior to (white) and 5 min after addition of cadaverine (grey and on side indicated by +). In Cre- fish, significant differences in repulsive behavior were detected after addition of cadaverine [6 min (p = 0.0084), 7 min (p = 0.0012), 8 min (p = 0.0017), 9 min (p = 0.0353) compared to the last min before addition, Wilcoxon signed-rank test, n = 15 fish]. Cre+ fish, did not show significant differences in their preferred location beyond two mins after cadaverine addition [6 min (p = 0.0302), 7 min (p = 0.0043) compared to the last min before addition, Wilcoxon signed-rank test, n = 15 fish]. (B) Preference index before and after addition of cadaverine averaged over 5 min periods for without [Cre-; –0.1341 ± 0.0923 and –0.6315 ± 0.0718] or with the Cre transgene [Cre+; –0.0823 ± 0.0631 and –0.3952 ± 0.1365], n = 15 fish per group. Two-way ANOVA reveals significant effect of time [F_{(3, 179)} = 32.45, p < 0.0001], group [F_{(14, 60)} = 3.4887, p = 0.0004] and interaction [F_{(42, 180)} = 4.887, p < 0.0001]. Post-hoc analysis by Bonferroni’s multiple comparisons. Dashed red lines in A and B denote midpoint of test tank.