Figure 3—figure supplement 2.

Validation of intersectional strategy to inhibit cholinergic neurons using botulinum neurotoxin.

Lateral views of (A) Tg(Xla.Tubb:QF2), Tg(QUAS:loxP-mCherry-loxP-BoTxBLC-GFP) and (B) Tg(Xla.Tubb:QF2), Tg(slc5a7a:Cre), Tg(QUAS:loxP-mCherry-loxP-BoTxBLC-GFP) larvae at 4 dpf. In the presence of Cre recombinase, cholinergic neurons in the spinal cord switch from mCherry to BoTxBLC-GFP expression, which inhibits their response to touch (refer to Video 1). Scale bars, 100 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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