FIGURE SUMMARY
Title

The Na(+)/PO(4) cotransporter SLC20A1 gene labels distinct restricted subdomains of the developing pronephros in Xenopus and zebrafish embryos

Authors
Nichane, M., Van Campenhout, C., Pendeville, H., Voz, M.L., and Bellefroid, E.J.
Source
Full text @ Gene Expr. Patterns
Fig. 1

SLC20A1 expression in Xenopus laevis embryos. Whole-mount in situ hybridization with the indicated DIG-labeled antisense probes was performed on embryos at the indicated stages (Nieuwkoop and Faber, 1997). (A–C) Lateral views of embryos with anterior towards the left. (D–I) High magnification views of the pronephric region. Arrows indicate SLC20A1 expression in the late distal tubule compartment of the developing pronephros. Abbreviations: dl, distal late pronephric compartment; de, distal early pronephric compartment; e, eye; nc, neural crest; np, neural plate; p, proximal pronephric compartment; s, somites.

Fig. 2

SLC20A1 expression during zebrafish embryogenesis. Whole-mount in situ hybridization with the indicated DIG-labeled antisense probes at the indicated stages. (A,B,E,G–J) Lateral views with anterior towards the right. (C,D) Transversal sections of the embryo shown in B at the levels indicated. (F) Dorsal view. (K,L) Costaining for NBC1 or ClC-K and ret1. ClC-K posterior limit of expression extends further than that of NBC1 and appears to correspond to the anterior limit of ret1. (M–O). Double in situ hybridization with ClC-K (green) and PDZK1 (red) indicates that a cell population in the middle of the pronephros expresses both ClC-K and PDZK1 (yellow). Arrowheads show the distinct posterior limit of the expression of the indicated markers. Abbreviations: cv, caudal vein; pcv, posterior caudal vein; pnd, pronephric duct segment; s, somites.

Fig. 3

Summary of the expression domains of the transporters used in this study in Xenopus and zebrafish pronephros. Xenopus pronephros has proximal and distal domains subdivided into early (E) and late (L) segments (Zhou and Vize, 2004). The zebrafish pronephric duct has been subdivided into four segments (S1–S4) based on the data presented here and previous unpublished results showing that ClC-K extends further anteriorly than ret1 in the zebrafish pronephros. S1 and S2 may correspond to proximal tubules. S3 expresses both proximal and distal tubule markers. S4 appears equivalent to the duct. The localization of the expression domains of the transporters is shown below the corresponding segments.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Gene expression patterns : GEP, 6(7), Nichane, M., Van Campenhout, C., Pendeville, H., Voz, M.L., and Bellefroid, E.J., The Na(+)/PO(4) cotransporter SLC20A1 gene labels distinct restricted subdomains of the developing pronephros in Xenopus and zebrafish embryos, 667-672, Copyright (2006) with permission from Elsevier. Full text @ Gene Expr. Patterns
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.