FIGURE

Fig. 5

ID

ZDB-FIG-240618-60

Publication

Sobah et al., 2024 - Stat3 regulates developmental hematopoiesis and impacts myeloid cell function via canonical and non-canonical modalities

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Fig. 5

Effect of Stat3 mutations on the response to LPS. Wild-type (WT: a, d, g, j), knockout (KO: b, e, h, k) and transactivation domain truncation (?TAD: c, f, i, l) mutant 3 dpf embryos on either the Tg(mpx:GFP) (a?f) or Tg(mpeg1.1:GFP) (g-l) transgenic background were left uninjected (a?c, g?i) or subjected to injection with lipopolysaccharide (LPS) (d?f, j?k) and imaged 8 h post injection showing representative images with scale bars of 100 ?m. Quantitation of mpx+ cells (m) and mpeg1.1+ cells (n) showing values for individual embryos. Gene expression analysis of the indicated genes in LPS-injected WT, KO and ?TAD embryos (n = 6) presented as fold-change (log2) relative to uninjected (o). Show mean and SEM with statistical significance indicated between genotypes (m, n) or compared to WT (o) (***p < 0.001, *p < 0.05, ns: not significant), or between uninjected and injected (##p < 0.01, #p < 0.05).

Expression Data

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Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Innate Immun.