FIGURE

Fig. 3

ID

ZDB-FIG-240618-58

Publication

Sobah et al., 2024 - Stat3 regulates developmental hematopoiesis and impacts myeloid cell function via canonical and non-canonical modalities

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Fig. 3

Effect of Stat3 mutations on early definitive hematopoiesis. Representative images of wild-type (WT), knockout (KO), and transactivation domain truncation (ΔTAD) mutant embryos subjected to WISH at 3.5 dpf with cmyb (a-c, e-g) and ikzf1 (i-k) and at 5 dpf with rag1 (m-o), tcra (q-s), hbbe1.1 (u-w), mpx (y-a′), and csf3r (c′-e′) using light microscopy or those on a Tg(mpeg1.1:GFP) transgenic background at 5 dpf (g′-i′) using fluorescence microscopy. Scale bars represent 200 μm. Quantification of area of staining for cmyb (d, h), ikzf1 (l), rag1 (P), tcra (t), and hbbe1.1 (x), and cell numbers for mpx (b′), csf3r (f′), and mpeg1.1 (j′) showing values for individual embryos as well as mean and SEM with statistical significance between genotypes indicated (***p < 0.001, **p < 0.01, *p < 0.05, ns: not significant).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Innate Immun.