|
Fig. 3 Effect of Stat3 mutations on early definitive hematopoiesis. Representative images of wild-type (WT), knockout (KO), and transactivation domain truncation (ΔTAD) mutant embryos subjected to WISH at 3.5 dpf with cmyb (a-c, e-g) and ikzf1 (i-k) and at 5 dpf with rag1 (m-o), tcra (q-s), hbbe1.1 (u-w), mpx (y-a′), and csf3r (c′-e′) using light microscopy or those on a Tg(mpeg1.1:GFP) transgenic background at 5 dpf (g′-i′) using fluorescence microscopy. Scale bars represent 200 μm. Quantification of area of staining for cmyb (d, h), ikzf1 (l), rag1 (P), tcra (t), and hbbe1.1 (x), and cell numbers for mpx (b′), csf3r (f′), and mpeg1.1 (j′) showing values for individual embryos as well as mean and SEM with statistical significance between genotypes indicated (***p < 0.001, **p < 0.01, *p < 0.05, ns: not significant).