FIGURE

Fig. 6.

ID

ZDB-FIG-231002-109

Publication

Leid et al., 2023 - Deletion of taf1 and taf5 in zebrafish capitulate cardiac and craniofacial abnormalities associated with TAFopathies through perturbations in metabolism

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Fig. 6.

taf1 deletion recapitulates the corazoncito phenotype. (A) Brightfield images of control and taf1^{stl456/stl456} embryos at 96 hpf. (B) taf1 mRNA expression measured by in situ hybridization (left) and RT-PCR (right). N=6 per experimental group. (C) Quantification of pericardial area (left) and ejection fraction (right) in control (n=30) and taf1^{stl456/stl456} (n=12) embryos at 96 hpf. (D) Quantification of head-to-body ratio (left) and head-to-eye ratio (right) in control (n=21) and taf1^{stl456/stl456} (n=20) embryos at 96 hpf. (E) Alcian Blue staining of control and taf1 knockout (KO) embryos. (F) X-ray microscopy-generated images of control and taf1 KO embryos at 96 hpf (left). Quantification of brain volume (right). N=4 per experimental group. Br, brain (red), heart (blue). (G) Whole-mount in situ hybridization of crestin and sox10 mRNA expression in control and taf1 KO embryos at 96 hpf. (H) Whole-mount in situ hybridization of neuronal (neurog1) and brain region markers (six3b, Adcyap1b, zic2a).

Expression Data

Genes:	adcyap1b crestin neurog1 six3b sox10 taf1 zic2a
Fish:	WT taf1^{stl456/stl456}
Anatomical Terms:	embryonic structure forebrain head heart hindbrain midbrain nervous system
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	taf1^{stl456/stl456}
Observed In:	brain cranial skeletal system development embryonic structure forebrain heart hindbrain midbrain nervous system pericardium trabecula cranii
Stage:	Day 4

Phenotype Detail

Acknowledgments

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