FIGURE

Figure 6

ID

ZDB-FIG-230711-26

Publication

Lescouzères et al., 2023 - A multilevel screening pipeline in zebrafish identifies therapeutic drugs for GAN

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Figure 6

Filter II: Novel methodological analysis of automated imaging‐screening

Schematic overview of 48‐h‐old embryos for automated detection in 96 well‐plates, ROI segmentation and Rescan imaging. Representative images of NMJ staining (znp1: green; αBTX: α‐bungarotoxin: red) within the spinal cord of control larvae.

Representative images of the detection filters segmenting AChR clusters from α‐bungarotoxin staining (1), pMNs axon area from znp1 staining and NMJ overlapping compounds (2) within the spinal cord of control larvae.

Representative image of the smoothing filter to extract the dorsal spinal cord staining as the specific region of interest to individualize axons (3).

Enlarged pictures of the parameters (1′–3′) for the quantification of NMJ and pMN axons.

Data information: Scale bar represents a length of 1 mm (A, 5×), 500 μm (A, 20×), 200 μm (B), 100 μm (D).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.