FIGURE

Fig 3

ID

ZDB-FIG-230406-71

Publication

Hong et al., 2023 - Essential role of an ERV-derived Env38 protein in adaptive humoral immunity against an exogenous SVCV infection in a zebrafish model

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Fig 3

Subcellular localization analysis of Env38 in HEK293T and EPC cells.

(A-C) HEK293T cells were transfected with the recombinant expression plasmid of pEGFPN1-Env38 (0.6 μg/ml) for 48 h, and then fixed and stained with the cell membrane probe DiI (A), ER-tracker (B) or Golgi-tracker (C) and nuclei probe DAPI. (D and E) EPC cells were transfected with the recombinant expression plasmid of pEGFPN1-Env38 (0.6 μg/ml) for 24 h and stimulated with SVCV (100 TCID₅₀) (E) or not (D) for another 24 h, and then fixed and stained with the cell membrane probe DiI and nuclei probe DAPI. The blue, green, and red fluorescence showed DAPI-labeled nuclei, Env38–EGFP fusion protein, and DiI–labeled cell membrane or ER-tracker–labeled ER or Golgi-tracker–labeled Golgi apparatus. Fluorescence images were captured using Laser scanning confocal microscope (FV3000) with 60 × oil glass.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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