FIGURE

Fig 5

ID
ZDB-FIG-230406-73
Publication
Hong et al., 2023 - Essential role of an ERV-derived Env38 protein in adaptive humoral immunity against an exogenous SVCV infection in a zebrafish model
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Fig 5

Detection of Env38 in tissues and cells in response to SVCV infection.

(A) RT-qPCR analysis for the expression of env38 gene in spleen, head kidney, intestine, liver, skin, gill, brain and heart tissues of zebrafish stimulated with SVCV (105 TCID50) or mock PBS (control) for 7 days. The expression of env38 mRNA in spleen in PBS treatment was arbitrarily set at 1. Negative control group was treated with mock PBS. Each sample was obtained from at least 10 fish. (B) Cellular distribution of Env38 on leukocytes from spleen, head kidney and peripheral blood of zebrafish stimulated with SVCV (105 TCID50). The Env38+ APCs were sorted from the leukocytes by FCM and qualitatively examined by RT-PCR with a panel of cellular markers of various immune cells, including CSF-1R and FcεRIγ (markers of monocytes/macrophages), CD83 and CD80 (markers of dendritic cells), membrane IgM (markers of B lymphocytes), MHC-II (markers of antigen presenting cells) as well as TCR-α and TCR-β (markers of T lymphocytes). Total leukocyte sample was used as a control. (C) Flow cytometry analysis of the percentages of Env38+ cells and MHC-II+Env38+ cells in lymphatic and myeloid leukocytes from spleen, head kidney and peripheral blood of zebrafish stimulated with SVCV (105 TCID50) for 7 days. The numbers above the outlined areas indicated the percentage of cells in each group. In control groups, zebrafish were i.p. with the same amount of mock PBS. In the RT-qPCR assay, PCRs were run in combination with the endogenous β-actin control. Error bars represent SEM. (*p < 0.05; **p < 0.01; ***p < 0.001; ns, no significant difference).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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