Fig 3

(A-C) HEK293T cells were transfected with the recombinant expression plasmid of pEGFPN1-Env38 (0.6 μg/ml) for 48 h, and then fixed and stained with the cell membrane probe DiI (A), ER-tracker (B) or Golgi-tracker (C) and nuclei probe DAPI. (D and E) EPC cells were transfected with the recombinant expression plasmid of pEGFPN1-Env38 (0.6 μg/ml) for 24 h and stimulated with SVCV (100 TCID₅₀) (E) or not (D) for another 24 h, and then fixed and stained with the cell membrane probe DiI and nuclei probe DAPI. The blue, green, and red fluorescence showed DAPI-labeled nuclei, Env38–EGFP fusion protein, and DiI–labeled cell membrane or ER-tracker–labeled ER or Golgi-tracker–labeled Golgi apparatus. Fluorescence images were captured using Laser scanning confocal microscope (FV3000) with 60 × oil glass.