Figure 7

Analysis of Sspo localization and RF formation in 30dpf katnb1 mutant brains

(A–L) Representative maximum intensity Z-stack projections of confocal micrographs, acquired through dorsally oriented whole mount brains that were dissected from 21dpf katnb1^mh102/+ control (A, C–G; N = 9, n = 61) and katnb1^mh102/mh102 mutant (B, H–L; N = 12, n = 82) fish, and immunostained for polyglutamylated tubulin (green) and Sspo (magenta). Squares indicate regions of interest, chosen for higher magnification analyses of areas surrounding the fChP (C, H); SCO (D, I); TeO (E, J); rChP (F, K); and medulla spinalis (G, L). (C–L) Inverted, higher magnification images of Sspo immunostaining. (C–G) katnb1^mh102/+ control animals exhibit normal Sspo localization and RF is present (arrows). (H–L) katnb1^mh102/mh102 mutant images, exhibiting abnormal Sspo accumulation at the fChP and rChP (double arrowheads), and the presence of an intact RF (arrows). Scale bars, 100 μm (A & B) and 50 μm (C–L).