FIGURE

Figure 6

ID

ZDB-FIG-220827-19

Publication

Crouzier et al., 2022 - Morphological, behavioral and cellular analyses revealed different phenotypes in Wolfram syndrome wfs1a and wfs1b zebrafish mutant lines

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Figure 6

Relative mRNA and protein expression levels of larvae zebrafish ER stress factors in physiological condition at 5 dpf. mRNA levels were analyzed by qPCR and protein contents by western blot in (A, C) wfs1a^C825X and (B, D) wfs1b^W493X. zeif2α and Stain-free™ were used as controls in qPCR and western blot analysis, respectively. The expression levels of mRNA and protein are represented as the fold change from the control group. Data are expressed in mean ± SEM, n = 5–9 in each group. ^*P < 0.05, ^***P < 0.001; unpaired t-test.

Expression Data

Genes:	atf4a atf4b atf6 ddit3 eif2s1b hsp90b1 hspa5 sigmar1 xbp1
Antibodies:	Ab2-ddit3 Ab3-sigmar1 Ab6-eif2a Ab11-hspa5
Fish:	AB wfs1a^{sa11465/sa11465} (AB) wfs1b^{sa16422/sa16422} (AB)
Anatomical Term:	whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	wfs1a^{sa11465/sa11465} (AB) wfs1b^{sa16422/sa16422} (AB)
Observed In:	whole organism
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Hum. Mol. Genet.