FIGURE

Fig. 2

ID

ZDB-FIG-220701-50

Publication

Basheer et al., 2022 - In vivo impact of JAK3 A573V mutation revealed using zebrafish

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Fig. 2

Phenotypic analysis of zebrafish carrying the Jak3 A573V encoding allele. Embryos homozygous for Jak3 wild�type (jak3^+/+) or Jak3 A573V (jak3^ca/ca) or those heterozygous for Jak3 A573V (jak3^+/ca) were subjected to WISH with ikzf1 at 3.5 dpf (A?C) and 5 dpf (E?G), rag1 (I?K), lck (M?O), tcra (Q?S), lyz (U?W) and mpo (Y?A?) at 5 dpf, as well as O-dianisidine staining (C??E?) (6.3/3.2?�?magnification, scale bar?=?200 ?m) or their blood analyzed with Giemsa staining at 5 dpf (F??H?; e, erythrocyte, l, lymphocyte) (100?�?magnification, scale bar?=?10 ?m). Individual embryos were assessed for relative area of staining with ikzf1 (D, H), rag1 (L), lck (P) and tcr? (T), for individual embryos or total number of lyz+ ⁺(X) and mpo+ (B?) cells, or blood differential counts (I?) with the mean and SEM shown in red and level of statistical significance indicated (*p?<?0.05, **p?<?0.01, ***p?<?0.001, ns: not significant) (n?=?30 for WISH analysis and n?=?5 for blood counts)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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