FIGURE

Fig. 6

ID

ZDB-FIG-220628-45

Publication

Wiltbank et al., 2022 - Cd59 and inflammation regulate Schwann cell development

Other Figures

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Fig. 6

(A) Top panel: immunofluorescence (IF) showing MACs (C5b-9+C5b-8; magenta, indicated with white arrows) embedded in sox10:megfp-positive posterior lateral line nerve (pLLN) SC membranes (gray) at 55 hours post fertilization (hpf). White dotted box corresponds with inset of a single z-plane on the right showing that MACs are within SC membranes. (B) Imaris renderings showing MACs (C5b-9+C5b-8; orange, indicated with white arrows) embedded in sox10:megfp-positive pLLN SC membranes (cyan) at 55 hpf. (B’) Enlarged renderings show MAC puncta (orange, indicated with black arrows) embedded in the SC membranes (cyan). (C) Scatter plot of the number of MACs in SC membranes at 55 hpf (mean ± SEM: WT: 3.3 ± 0.3, cd59^uva48: 11.6 ± 1.9; p<0.0001; dot = 1 fish). These data were compared with Student’s t-test using GraphPad Prism. All data were normalized to units per 100 µm. All images were acquired with confocal imaging. Scale bars: (A, B) 10 µm; inset (A) and enlarged renderings (B’), 5 µm.

Expression Data

Gene:	EGFP
Antibody:	ab1-c5
Fish:	sl3Tg cd59a^uva48/uva48; sl3Tg
Anatomical Terms:	myelinating Schwann cell membrane attack complex posterior lateral line nerve myelinating Schwann cell
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cd59a^uva48/uva48; sl3Tg
Observed In:	myelinating Schwann cell activation of membrane attack complex
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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