FIGURE

FIGURE 1

ID

ZDB-FIG-220406-1

Publication

Zheng et al., 2022 - Kif15 Is Required in the Development of Auditory System Using Zebrafish as a Model

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FIGURE 1

Knockdown of Kif15 with splicing-blocking method inhibits the normal formation of vestibular. (A,B) The protein level of Kif15 is severely decreased in Kif15-morpholino (MO)-injected embryos both in the band intensity (A) and in the semiquantitative analysis (B), in comparison with control embryos. Data are recorded as mean ± SEM. **p < 0.01. (C–H) Kif15-MO suppresses the normal development of semicircular canals. The abnormal manifestations are observed at 48 hpf (C,F), 72 hpf (D,G), and 96 hpf (E,H), respectively. ap, anterior protrusion; ab, anterior bulge; vb, ventral bulge; vp, ventral protrusion; pb, posterior bulge; pp, posterior protrusion; u, utricle; and s, saccule. Red arrowheads show the abnormal fusion of anterior and posterior protrusions. (I–K) Co-injection using Kif15-MO and Kif15 mRNA successfully rescues the phenotypes of semicircular canals and otoliths at 48 hpf (I), 72 hpf (J), and 96 hpf (K), respectively. Scale bar is 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-kif15
Knockdown Reagent:	MO2-kif15
Observed In:	semicircular canal semicircular canal morphogenesis
Stage Range:	Long-pec to Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.