FIGURE

Fig 7

ID

ZDB-FIG-220318-7

Publication

Nandamuri et al., 2022 - Loss of zebrafish dzip1 results in inappropriate recruitment of periocular mesenchyme to the optic fissure and ocular coloboma

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Fig 7

<italic toggle='yes'>dzip1</italic> mutants exhibit defective accumulation of POM-derived endothelial cells.

(A-F) Embryos visualized for endothelial cells (green; Tg(kdrl:ras-mCherry)) and cell membranes (magenta; Tg(bactin2:EGFP-CAAX)) in wild type (A-C) and dzip1^ts294e mutants (D-F) at 24 hpf (A, D), 36 hpf (B, E), and 48 hpf (C, F). All images are lateral views of 3-dimensional renderings. mCherry-positive endothelial cells populate the optic fissure by 24 hpf in both wild type and dzip1^-/- embryos (A, D; white arrowheads). (E) asterisks, ectopic branching of the superficial network in dzip1 mutant. (G-I) Quantification of fissure vessel width (in μm) at 24 hpf, 36 hpf, and 48 hpf. n (embryos) for each genotype shown at the base of the graph. P-values were calculated using Welch’s t-test (M-O). Scale bar: 50 μm. D, dorsal; V, ventral; N, nasal; T, temporal.

Expression Data

Genes:	EGFP mCherry
Fish:	s896Tg; z200Tg dzip1^{ts294e/ts294e}; s896Tg; z200Tg
Anatomical Terms:	eye plasma membrane hyaloid vessel blood vessel endothelial cell periocular mesenchyme endothelial cell
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dzip1^{ts294e/ts294e}; s896Tg; z200Tg
Observed In:	hyaloid vessel ocular blood vessel
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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