ZFIN Image: Nandamuri et al., 2022, Fig 7

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Figure Caption

Fig 7 <italic toggle='yes'>dzip1</italic> mutants exhibit defective accumulation of POM-derived endothelial cells.

(A-F) Embryos visualized for endothelial cells (green; Tg(kdrl:ras-mCherry)) and cell membranes (magenta; Tg(bactin2:EGFP-CAAX)) in wild type (A-C) and dzip1^ts294e mutants (D-F) at 24 hpf (A, D), 36 hpf (B, E), and 48 hpf (C, F). All images are lateral views of 3-dimensional renderings. mCherry-positive endothelial cells populate the optic fissure by 24 hpf in both wild type and dzip1^-/- embryos (A, D; white arrowheads). (E) asterisks, ectopic branching of the superficial network in dzip1 mutant. (G-I) Quantification of fissure vessel width (in μm) at 24 hpf, 36 hpf, and 48 hpf. n (embryos) for each genotype shown at the base of the graph. P-values were calculated using Welch’s t-test (M-O). Scale bar: 50 μm. D, dorsal; V, ventral; N, nasal; T, temporal.

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Acknowledgments

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