Figure 2

Retinal vasculature and renal alterations in akr1a1a^−/− Tg(fli1:EGFP) zebrafish larvae and adults. A) Representative confocal images of the hyaloid vasculature in zebrafish larvae. White box: central hyaloid/optic artery. White arrow: circumferential inner annular hyaloid vessel. B) Representative confocal images of the hyaloid vasculature in akr1a1a^+/+ and akr1a1a^−/− larvae show vascular alterations in the mutants. White scale bar = 20 µm. C,D) Quantification of increased branches and sprout formation in the hyaloid vasculature in akr1a1a^−/− larvae, n = 10–16 per group. E) Representative confocal images of retinal vasculature in akr1a1a^+/+ and akr1a1a^−/− adults. White scale bar = 200 µm. F,G) Quantification of increased branches and sprout formation in the adult retinal vasculature in akr1a1a^−/− zebrafish, n = 13–16 per group. H,I) Representative electron microscopy images and quantification showed thicker GBM in adult akr1a1a^−/− kidneys. Red arrows: GBM. White scale bar, 500 nm. For statistical analysis Student's t‐test was applied. *p < 0.05, **p < 0.01.