ZFIN Figure: Hawkins et al., 2021, Fig. 5

Fig. 5

Figure 5. F-actin formation in the early fin fold is affected in wasl mutants and is associated with Hox13-positive cells and the actinotrichia

(A) 3 dpf phalloidin-labeled pectoral fins showing modified F-actin in reph homozygous mutants and wasla; waslb double mutants. reph homozygotes develop large F-actin aggregates and dysmorphic fin folds, and wasla; waslb double mutants display a more dispersed F-actin network.

(B) F-actin foci colocalize with the extensions of Hox13-positive cells in the fin fold marked with the Tg(2PΔepi:eGFP) reporter transgene.

(C) A single Tg(2PΔepi:eGFP) Hox13-positive cell showing F-actin colocalization.

(D) Triple labeling demonstrates colocalization of F-actin foci, Hox13-positive cells expressing the Tg(mInta-11:mCherry) transgene, and actinotrichia in the fin fold.

(E) A single Tg(mInta-11:mCherry) Hox13-positive cell showing colocalization with actinotrichia fibrils and F-actin.

Anterior to left, distal to top in all panels; scale bars (A) 100 μm (25 μm inset), (B, D) 50 μm, (C, E) 10 μm (2 μm inset in E).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	waslb^dmh22/dmh22 wasla^mh150/mh150; waslb^mh130/mh130
Observed In:	pectoral fin fold pectoral fin fold actin filament
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 184(4), Hawkins, M.B., Henke, K., Harris, M.P., Latent developmental potential to form limb-like skeletal structures in zebrafish, 899-911.e13, Copyright (2021) with permission from Elsevier. Full text @ Cell