FIGURE

Fig. 1

ID

ZDB-FIG-210325-46

Publication

Hawkins et al., 2021 - Latent developmental potential to form limb-like skeletal structures in zebrafish

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Fig. 1

Figure 1. Gain-of-function mutations reveal capacity for limb-like development in fins

(A) From a common ancestor, teleosts and tetrapods evolved divergent appendage patterns (see also Figure S1).

(B and C) Adult pectoral fin skeletons shown intact (left); fin rays and distal radials removed (center); schematized (right) (see also Figure S2). (B) Wild-type pectoral skeleton. (C) Heterozygous reph mutant pectoral skeleton.

(D) waslb and vav2 alleles causing mutant phenotypes; Δ+ reph (mh130), and Δ+ wan (mh138) rescue wild-type patterning (see also Figure S3).

(E) Epistasis analysis showing suppression of wan/vav2 phenotypes after loss of waslb function (see also Figure S4).

(F) Genetic pathway of vav2, waslb, and fin elaboration.

(G) Expression of waslb and vav2 in 48 hpf pectoral fin buds.

Anterior to left, distal to top; scale bars (B, C, E) 500 μm, (G) 50 μm.

Expression Data

Genes:	vav2 waslb
Fish:	WT
Anatomical Term:	pectoral fin bud
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vav2^ty127/+ waslb^dmh22/+ waslb^mh130/mh130; vav2^ty127/+
Observed In:	pectoral fin pectoral fin radial
Stage:	Adult

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 184(4), Hawkins, M.B., Henke, K., Harris, M.P., Latent developmental potential to form limb-like skeletal structures in zebrafish, 899-911.e13, Copyright (2021) with permission from Elsevier. Full text @ Cell