FIGURE

Figure 1

ID

ZDB-FIG-210319-63

Publication

Lai et al., 2021 - MicroRNA-21 Plays Multiple Oncometabolic Roles in the Process of NAFLD-Related Hepatocellular Carcinoma via PI3K/AKT, TGF-β, and STAT3 Signaling

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Figure 1

Generation of a liver specific and inducible microRNA (miR)-21 transgenic zebrafish. (A) Schematic diagram of the DNA construct used to generate LmiR21 [Tg(fabp10a-Tet^on-2A-ZsGreen:mCherry-miR-21)] transgenic zebrafish. Fabp10a promoter drives expression of ZsGreen and tetracycline-inducible transcription factor (Tet-on/off), which controls mCherry and miR-21 expression. (B) Relative quantification of miR-21 expression using (RT-qPCR) analysis. LmiR21#1-5+doxycycline (Dox) represent five independent transgenic lines. Control: wild-type (WT)-Dox and LmiR21#1-5-Dox zebrafish. (C) Liver-specific inducible miR-21 expression in the LmiR21#1 7 days post fertilization (dpf). Transgenic larvae were treated with 25 μg/mL Dox from 48 or 72 h post fertilization (hpf) to 7 dpf. Scale bar: 100 μm. (D) Left: images of zebrafish liver; right: 2D measurements of liver area using ImageJ. Scale bar: 50 μm. (E) Representative photomicrographs of IHC detection of cell proliferation marker-PCNA in zebrafish liver (Left). Scale bar: 50 μm; Statistical analysis of the percentage of PCNA-positive field. Statistically significant differences from −Dox group were denoted by * (p < 0.05), ** (p < 0.01) and *** (p < 0.001) for panel D and E.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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