FIGURE

Figure 1

ID

ZDB-FIG-210319-26

Publication

Lai et al., 2021 - MicroRNA-21 Plays Multiple Oncometabolic Roles in the Process of NAFLD-Related Hepatocellular Carcinoma via PI3K/AKT, TGF-β, and STAT3 Signaling

Other Figures

All Figure Page

Back to All Figure Page

Figure 1

Generation of a liver specific and inducible microRNA (miR)-21 transgenic zebrafish. (A) Schematic diagram of the DNA construct used to generate LmiR21 [Tg(fabp10a-Tet^on-2A-ZsGreen:mCherry-miR-21)] transgenic zebrafish. Fabp10a promoter drives expression of ZsGreen and tetracycline-inducible transcription factor (Tet-on/off), which controls mCherry and miR-21 expression. (B) Relative quantification of miR-21 expression using (RT-qPCR) analysis. LmiR21#1-5+doxycycline (Dox) represent five independent transgenic lines. Control: wild-type (WT)-Dox and LmiR21#1-5-Dox zebrafish. (C) Liver-specific inducible miR-21 expression in the LmiR21#1 7 days post fertilization (dpf). Transgenic larvae were treated with 25 μg/mL Dox from 48 or 72 h post fertilization (hpf) to 7 dpf. Scale bar: 100 μm. (D) Left: images of zebrafish liver; right: 2D measurements of liver area using ImageJ. Scale bar: 50 μm. (E) Representative photomicrographs of IHC detection of cell proliferation marker-PCNA in zebrafish liver (Left). Scale bar: 50 μm; Statistical analysis of the percentage of PCNA-positive field. Statistically significant differences from −Dox group were denoted by * (p < 0.05), ** (p < 0.01) and *** (p < 0.001) for panel D and E.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cancers