FIGURE

Fig. 2

ID

ZDB-FIG-210218-101

Publication

Cao et al., 2021 - Calcineurin controls proximodistal blastema polarity in zebrafish fin regeneration

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Fig. 2

The PCE failed to form blastemas. (A) In situ hybridization of and1/2, raldh2, msxb, runx2b, and lef1 in regenerative fins at 2 dpa showing that blastemas were not built in the PCE. (B) and1 and lef1 were not expressed in the PCE with two-hole excavations. (C) Histological analyses of ACE (n = 6/8) and PCE (n = 5/6). (D) H3P antibody staining (red) was used to examine cell proliferation in the ACE (n = 7/10) and PCE (n = 7/9) DAPI counterstaining (blue). (E) qRT-PCR results comparing the differences in the relative expression of regeneration-associated genes for the PCE over the ACE (Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001). Data are presented as mean ± SD. P, proximal; D, distal. (Scale bars: 200 µM in A and B; 100 µM in C and D.)

Expression Data

Genes:	aldh1a2 and1 and2 cyp26a1 fgf20a lef1 msx1b rarga runx2b sparc wnt5a wnt10a
Fish:	WT
Condition:	amputation: caudal fin
Anatomical Term:	blastema proximal-distal axis
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	amputation: caudal fin
Observed In:	regenerating fin cell division
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA