FIGURE

Figure 8

ID

ZDB-FIG-210119-32

Publication

Ohta et al., 2020 - Emx2 regulates hair cell rearrangement but not positional identity within neuromasts

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Figure 8

(A) Maximum intensity projection and 3-D rendering of selected images of nascent sibling HCs in myo6b:Yfp-tubulin larvae (magenta and green dots) that was treated with nocodazole for 90 min immediately after the precursor divided. The apical protrusion that normally forms within 18 min was still evident initially (18 min; red and white arrowheads) but disappeared quickly between 30 and 36 min (asterisks, 48 min). After nocodazole removal, the apical protrusion reappeared after 12 min (102 min, red and white arrowheads) and the two nascent HCs rolled to their respective positions after 84 min (186 min). (B) A nascent HC pair (red and green dots) that was treated with nocodazole for 150 min immediately after the precursor divided. Similar to 90 min treatments, the apical protrusion was evident between 12 and 18 min (12 min, red and white arrowheads) after HC precursor divided (0 min) but disappeared shortly within 30–36 min (54 min, asterisk). However, after nocodazole removal at 150 min, sibling HCs did not form an apical protrusion and exchange positions (162–240 min, asterisks).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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