PUBLICATION

Emx2 regulates hair cell rearrangement but not positional identity within neuromasts

Authors
Ohta, S., Ji, Y.R., Martin, D., Wu, D.K.
ID
ZDB-PUB-210101-10
Date
2020
Source
eLIFE   9: (Journal)
Registered Authors
Keywords
developmental biology, zebrafish
Datasets
GEO:GSE152859
MeSH Terms
  • Animals
  • Cell Polarity/physiology
  • Gene Expression Regulation, Developmental/physiology*
  • Hair Cells, Auditory/metabolism*
  • Homeodomain Proteins/metabolism*
  • Lateral Line System/physiology
  • Transcription Factors/metabolism*
  • Zebrafish/metabolism
PubMed
33377867 Full text @ Elife
Abstract
Each hair cell (HC) precursor of zebrafish neuromasts divides to form two daughter HCs of opposite hair bundle orientations. Previously, we showed that transcription factor Emx2, expressed in only one of the daughter HCs, generates this bidirectional HC pattern (Jiang et al., 2017). Here, we asked whether Emx2 mediates this effect by changing location of hair bundle establishment or positions of HCs since daughter HCs are known to switch positions with each other. We showed this HC rearrangement, redefined as two processes named Rock & Roll, is required for positional acquisition of HCs. Apical protrusion formation of nascent HCs and planar polarity signaling are both important for the Rock & Roll. Emx2 facilitates Rock & Roll by delaying apical protrusion of its nascent HCs but it does not determine HCs' ultimate positions, indicating that Emx2 mediates bidirectional HC pattern by changing the location where hair bundle is established in HCs.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping