FIGURE

Figure 2.

ID

ZDB-FIG-210119-22

Publication

Ohta et al., 2020 - Emx2 regulates hair cell rearrangement but not positional identity within neuromasts

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Figure 2.

Emx2 immunostaining in wild-type (WT) (<italic>myo6b:actb1-Gfp</italic>) neuromasts.

(A and D) Merged image of DAPI, (B and E) β-actin-GFP, and (C and F) anti-Emx2 staining of neuromasts. Emx2 immunoreactivity is not detected in dividing hair cell (HC) precursor (#1, arrowhead) or daughter HCs during Rock and Roll (#2). However, it is detectable in immature daughter HCs after Rock and Roll, in which the oriented hair bundle is not yet evident (#3, E, magenta arrows), as well as in mature HCs (#4). Both immature (#3) and mature HCs (#4, A→P) that are located in the anterior side of the neuromast are Emx2-positive. Insets in B and E are higher magnifications showing the hair bundle orientation pattern at the apex of HCs.

Expression Data

Gene:	emx2
Antibody:	Ab1-emx2
Fish:	vo8Tg
Anatomical Terms:	neuromast anterior region neuromast hair cell
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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