FIGURE

Figure 4

ID

ZDB-FIG-201229-65

Publication

Keefe et al., 2020 - Vanishing white matter disease expression of truncated EIF2B5 activates induced stress response

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Figure 4

Top row (and quantification in lower left): increased apoptotic OPCs in the brain and spinal cord. Confocal images of brain, z-stack, rostral to the top, double-labeling for TUNEL and olig1 (in situ probe), in WT, eif2b5^zc103/zc103, eif2b5^zc102/zc102 or eif2b2^{sa17223/sa172233} larvae. Region used for quantification shown by box. Inset in each panel shows example of double-labeled cell (TUNEL, olig1) for each genotype (except WT), single confocal slice image. Middle panels: no change in apoptosis of differentiated oligodendrocytes, co-labeled with myelin associated glycoprotein (mag) (in situ probe) and TUNEL. Confocal z-stack images of spinal cord, rostral to the left; quantified in lower right panel.

Expression Data

Genes:	mag olig1
Fish:	WT eif2b2^{sa17223/sa17223} eif2b5^zc102/zc102 eif2b5^zc103/zc103
Anatomical Terms:	hindbrain glioblast (sensu Vertebrata) hindbrain oligodendrocyte spinal cord glioblast (sensu Vertebrata) spinal cord oligodendrocyte
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	eif2b2^{sa17223/sa17223} eif2b5^zc102/zc102 eif2b5^zc103/zc103
Observed In:	brain apoptotic process glioblast apoptotic process hindbrain glioblast oligodendrocyte apoptotic process spinal cord glioblast
Stage:	Day 5

Phenotype Detail

Acknowledgments

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