FIGURE

Fig. 4.

ID

ZDB-FIG-200904-4

Publication

Lavergne et al., 2020 - Pancreatic and intestinal endocrine cells in zebrafish share common transcriptomic signatures and regulatory programmes

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Fig. 4.

Expression profiles of pax4, arx, pdx1 and fev in the zebrafish gut. Fluorescent in situ hybridization (FISH) performed on 66 hpf (a), 72 hpf (b–d) or 96 hpf (e–i) embryos. Views of the gut (delimited by dotted lines; anterior to the left) showing the non-overlapping expression of pax4 (green) and arxa (red) (a), numerous EECs expressing fev (b) and insm1b (d). Higher magnification showing insm1+ EECs located within the gut epithelium and insm1+ enteric neurons (shown by asterisks) outside but juxtaposed to the epithelium. e View of the gut from the tg(BAC pdx1:GFP) larvae (GFP immunostaining) with higher magnification of the anterior (f) and posterior (g) parts. FISH using pdx1 probe (h) and immunostaining using Pdx1 antibody. P, pancreas. Scale bars, 20 μM

Expression Data

Genes:	arxa fev GFP insm1b pax4 pdx1
Antibody:	Ab2-pdx1
Fish:	AB bns13Tg
Anatomical Terms:	enteric neuron enteroendocrine cell gut intestinal bulb intestine intestine enteroendocrine cell mid intestine enteroendocrine cell pancreas posterior intestine enteroendocrine cell
Stage Range:	Pec-fin to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Biol.