FIGURE

Fig 3

ID

ZDB-FIG-200829-81

Publication

Zhang et al., 2020 - THOC1 deficiency leads to late-onset nonsyndromic hearing loss through p53-mediated hair cell apoptosis

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Fig 3

<italic>Thoc1</italic> deficiency caused hair cell developmental defects in zebrafish.

(a) Fluorescence microscopic imaging analysis of thoc1 mutant Tg(pou4f3:gap43-GFP) line at 3 dpf. Arrowheads indicate hair cell clusters. (b) Statistical analysis of the hair cell clusters in control and thoc1 mutants (control, n = 10; thoc1 mutants, n = 37). t-test, ****, p<0.0001. (c) Fluorescence microscopic imaging analysis of thoc1 mutant Tg(pou4f3:gap43-GFP) line at 4 dpf. Arrowheads indicate hair cell clusters. (d) Statistical analysis of the hair cell clusters in control and thoc1 mutants (control, n = 10; thoc1 mutants, n = 38). t-test, ****, p<0.0001. (e) Confocal microscopic imaging analysis of the neuromasts in control and thoc1 mutants at 3 dpf. Green: Tg(pou4f3:gap43-GFP). Red: Sox2 antibody staining marking supporting cells. Blue: DAPI staining of the cell nuclei. (f, g) Statistical analysis of the hair cell number per neuromasts and total number in trunk of control and thoc1 mutants (control, n = 9; thoc1 mutants, n = 17, control, n = 15; thoc1 mutants, n = 15) at 3 dpf. t-test, ****, p<0.0001. (h) Confocal microscopic imaging analysis of the neuromasts in control and thoc1 mutants at 4 dpf. Green: Tg(pou4f3:gap43-GFP). Red: Sox2 antibody staining marking supporting cells. Blue: DAPI staining of the cell nuclei. (i, j) Statistical analysis of the hair cell number per neuromasts and total number in trunk of control and thoc1 mutants (control, n = 9, thoc1 mutants, n = 17; control, n = 15; thoc1 mutants, n = 15) at 4 dpf. t-test, ****, p<0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s356tTg + CRISPR1-thoc1
Knockdown Reagent:	CRISPR1-thoc1
Observed In:	neuromast neuromast hair cell
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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